Study on spatio-temporal prevalence and hematological attributes of bovine Babesiosis in cattle population of Layyah, Southern Punjab, Pakistan

ABSTRACT The present study was conducted to investigate the spatiotemporal prevalence of bovine babesiosis in cattle population (n=376) Layyah, Pakistan as affected by location, age, breed, gender, and seasons. Blood samples were collected aseptically assessed for through microscopy PCR, automated analyzer hematological attributes. Overall results PCR showed significantly (P≤0.05) lower 19.4% (n=72/376) compared 37.2% (n=140/276) microscopy. None studied from Cholistani breed Babesia-positive. However, higher noticed crossbred (46.7%, n= 50/107) followed that Friesian (16.1%, 10/62), Jersey (7%, 5/71) Sahiwal (6.9%, 7/101) breeds. Female (19.5%, 55/281) age group 1 (Up 2 years) (40%, 42/105) had Babesia ascertained comparison their counterpart groups. Significantly 35.9% (n=60/167) shown summer winter season (5.7%, 12/209). All positive produced 490bp amplicons specific typical bigemina. Hemoglobin concentration, erythrocytic count, hematocrit mean corpuscular volume babesia-positive healthy ones.